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 · 26.08.2021. Primetals Technologies to supply a new bar rolling mill to Pak Steel Pakistan. Find out more 25.08.2021. Hydrogen-based ironmaking MHI Australia and Primetals Technologies join Heavy Industry Low-Carbon Transition Cooperative Research Centre. Find out more

Green fluorescent protein (GFP) applications in cell

 · The two most commonly used red-shifted GFP variants are Ser65Thr (18 22) which contains a Ser65 to Thr substitution in the chromophore and GFPmut1 or enhanced green fluorescent protein (EGFP)23 24 which contains the same Ser65Thr change plus a Phe64Leu mutation.Advantages of EGFP for applications in mammalian cells include improved sensitivity of detection improved

An Arabidopsis AT-hook motif nuclear protein mediates

 · Using the centromere-specific HISTONE3-GFP fusion protein (CENH3-GFP) 41 42 seven to eight CENH3-GFP-marked centromeres could be detected in root cells of wild-type

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Enhanced snoMEN Vectors Facilitate Establishment of GFP

 · The snoMEN (sno RNA M odulator of gene E xpressio N) vector technology was developed from a human box C/D snoRNA HBII-180C which contains an internal sequence that can be manipulated to make it complementary to RNA targets allowing knock-down of targeted genes.Here we have screened additional human nucleolar snoRNAs and assessed their application for gene specific

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High throughput generation of promoter reporter (GFP

 · Although the complete genome sequence and annotation of Arabidopsis were released at the end of year 2000 it is still a great challenge to understand the function of each gene in the Arabidopsis genome. One way to understand the function of genes on a genome-wide scale is expression profiling by microarrays. However the expression level of many genes in Arabidopsis genome cannot be detected

Bioluminescence of AequoreaUniversity of Washington

 · Bioluminescence and other factoids about Aequorea a hydromedusa. Aequorea victoria is a jellyfish in Puget Sound Washington State from which the luminescent protein aequorin and the fluorescent molecule GFP (green fluorescent protein) have been extracted purified and eventually cloned. These two products have proven useful and popular in various kinds of biomedical research in

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Two-color GFP expression system for C. elegans

A yellow-shifted GFP derivative designated YFP contains aa substitutions S65G V68A S72A and T203Y and is similar to the previously described "I0C" variant. Coding regions for CFP and YFP were constructed in the context of a high-activity C. elegans expression system.

Enhanced snoMEN Vectors Facilitate Establishment of GFP

 · Plasmid vectors encoding mCherry linked either to wild type snoRNAs or linked to snoMEN targeted to GFP mRNA were transiently transfected in HeLa cell lines that stably express GFP-SMN1 (HeLa GFP-SMN) (Fig 4A and Fig F in S1 File). Expression of mCherry was used as a marker for detecting transfected cells.

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THE GREEN FLUORESCENT PROTEIN Annual Review of

 · In just three years the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal

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SOX3 is required during the formation of the hypothalamo

 · (d) The live GFP activity domain in a 12.5-d.p.c. heterozygous (XX /−) embryo was absent in a wild-type (XY ) embryo but it matched the area of normal Sox3 expression by in situ hybridization

How to Use the Government Furnished Property

 · 4 Types of GFP Attachment Formats Requisitioned Government-Furnished Property (RGFP) » Lists Government Property to be authorized for contractor requisition from DoD supply sources in accordance with the clause at FAR 52.251-1. Scheduled Government-Furnished Property (SGFP) » Lists the Government property to be provided including when major end items are being provided

Survival of gfp-tagged antagonistic bacteria in the

 · Introduction. Ralstonia solanacearum is a soil-borne pathogen that naturally infects roots and invades and multiplies in the xylem vessels.Ralstonia solanacearum has an extremely wide host range with over 200 plant species belonging to more than 50 botanical families and is a major plant pathogen in subtropical and tropical areas.Ralstonia solanacearum (biovar 2/race 3) which causes

Green fluorescent protein (GFP) applications in cell

 · The two most commonly used red-shifted GFP variants are Ser65Thr (18 22) which contains a Ser65 to Thr substitution in the chromophore and GFPmut1 or enhanced green fluorescent protein (EGFP)23 24 which contains the same Ser65Thr change plus a Phe64Leu mutation.Advantages of EGFP for applications in mammalian cells include improved sensitivity of detection improved

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A monocot-specific hydroxycinnamoylputrescine gene

 · A previous study detected a single locus on chromosome 9 that is related to the production of Fer-Put .Bioinformatic analysis of the genomic region identified a gene encoding ODC (OsODC).Phylogenetic analysis revealed high sequence similarity between the OsODC protein and other ODCs in plants and animals (Fig. S1 online) terestingly two tandem-duplicated genes encoding PHTs LOC

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Patterning and growth control in vivo by an engineered

 · Morphogens provide positional information during tissue development. For this behavior to occur morphogens must spread out and form a concentration gradient however their mechanism of transport remains a matter of debate. Stapornwongkul et al. now show that in the presence of extracellular binding elements (binders) the inert green fluorescent protein (GFP) can form a

GFP sheds light on the infection process of avocado roots

 · Pathogenicity test in avocado plants of R. necatrix isolates CH53 and CH53-GFP. Aerial symptoms developed on 24-month-old avocado plants (A) and the evaluation of the disease symptoms on avocado seedlings (B) 2 months after inoculation of the indicated strains. Control plants were exposed to non-infected wheat grains.

GFP-based optimization scheme for the overexpression

A monocot-specific hydroxycinnamoylputrescine gene

 · A previous study detected a single locus on chromosome 9 that is related to the production of Fer-Put .Bioinformatic analysis of the genomic region identified a gene encoding ODC (OsODC).Phylogenetic analysis revealed high sequence similarity between the OsODC protein and other ODCs in plants and animals (Fig. S1 online) terestingly two tandem-duplicated genes encoding

A General Mechanism of Green-to-Red Photoconversions

 · Here we dissect the phenomena of oxidative and reductive green-to-red photoconversion of the Green Fluorescent Protein. We characterize distinct orange- and red-emitting forms (λabs/λem = 490/565 nm λabs/λem = 535/600 nm) arising during the Enhanced Green Fluorescent Protein (EGFP) photoconversion under low-oxygen conditions in the presence of reductants.

THE GREEN FLUORESCENT PROTEIN Annual Review of

 · In just three years the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal

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Comparative AAV-eGFP Transgene Expression Using

Residual stress analyses in multilayer PP/GFP/PP

 · The yield stress depends on the type of specimen used and the respective values for type 1 type 2 and type 3 (see Fig. 1) have been recorded as 21.6 26.2 and 17.3 MPa and the corresponding tensile strength as 25.19 27.14 and 23.23 MPa. However in the outer double layer PP/GFP and the inner double layer GFP/PP as soon as the plastic

SOX3 is required during the formation of the hypothalamo

 · (d) The live GFP activity domain in a 12.5-d.p.c. heterozygous (XX /−) embryo was absent in a wild-type (XY ) embryo but it matched the area of normal Sox3 expression by in situ hybridization

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PDB101 Molecule of the Month Green Fluorescent

GFP is a ready-made fluorescent protein so it is particularly easy to use. Most proteins that deal with light use exotic molecules to capture and release photons. For instance the opsins in our eyes use retinol to sense light (see the Molecule of the Month on bacteriorhodopsin). These "chromophores" must be built specifically for the task

Expression and Subcellular Distribution of GFP-Tagged

 · GFP fluorescence emission was measured at 512 nm by excitation at 488 nm using Infinite M200PRO plate reader (Tecan). Glass beads were added to a final volume of 500 μl and an additional 500 μl YSB added next cells were broken with a mixer-mill disruptor.

How to Use the Government Furnished Property

 · 4 Types of GFP Attachment Formats Requisitioned Government-Furnished Property (RGFP) » Lists Government Property to be authorized for contractor requisition from DoD supply sources in accordance with the clause at FAR 52.251-1. Scheduled Government-Furnished Property (SGFP) » Lists the Government property to be provided including when major end items are being provided under a

A role for the small GTPase Rab21 in the early endocytic

 · (A-C) Lysates from HeLa cells expressing GFP-Rab21 wt GFP-Rab21 T33N GFP-Rab21 Q78L or GFP alone were immunoprecipitated using anti-GFP antibodies and subjected to SDS-PAGE and western analysis before detection of GFP by ECL (A) or GTP binding using 32 P-GTP (B). Graph in C shows quantification of radioactivity from GFP-Rab21 bands in B and

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GFP-based optimization scheme for the overexpression

 · Construction of GFP-8His-containing vector. Timing 2–3 weeks. 1. Clone the sequence for yEGFP 15 (less start Methionine) with a C-terminal octa-His tag and a TEV protease site upstream of GFP

A General Mechanism of Green-to-Red Photoconversions